The Women's 25 km Open Water event at the 2007 World Aquatics Championships was held on March 25, 2007 at St Kilda Beach.
| # | Name | Country | Time | Pts |
|---|---|---|---|---|
| 1 | Britta Kamrau-Corestein | Germany | 5:37:11.66 | 18 |
| 2 | Kalyn Keller | United States | 5:39:39.62 | 16 |
| 3 | Ksenia Popova | Russia | 5:39:51.51 | 14 |
| 4 | Angela Maurer | Germany | 5:40:00.13 | 12 |
| 5 | Natalia Pankina | Russia | 5:40:01.87 | 10 |
| 6 | Jana Pechanová | Czech Republic | 5:47:23.28 | 8 |
| 7 | Shelley Clark | Australia | 5:47:24.88 | 6 |
| 8 | Laura la Piana | Italy | 6:07:21.71 | 5 |
| 9 | Malwina Bukszowana | Poland | 6:11:31.54 | 4 |
| 10 | Evelien Sohl | Netherlands | 6:24:25.82 | 3 |
| Alessandra Romiti | Italy | DNF | ||
| Darija Pop | Montenegro | DNF | ||
| Eva Berglund | Sweden | DNF | ||